Electrophoretic behaviour of human serum albumin at pH 4
نویسندگان
چکیده
منابع مشابه
Electrophoretic Behavior of Normal Human Serum Albumin at pH 4.0
In the preceding paper on the behavior of pooled normal human serum albumin in various buffers at pH 4.0 (1)) it was demonstrated that the electrophoretic pattern of albumin shows a single moving boundary in the presence of the lower fatty acids, excepting formic and acetic acid, and of certain diand tribasic organic acids. This phenomenon is probably a consequence of the interaction between th...
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In previous publications from this laboratory, we have investigated the anomalous behavior of human serum albumin (l), various animal albumins (2) and human -y-globulin (3) in the Tiselius electrophoresis apparatus at acid pH. Because of increasing interest in the behavior of proteins in the acid region below their isoelectric point, this phenomenon has been receiving increasing attention from ...
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Recently we have reported on a hitherto unknown modification of human serum albumin of which the outstanding properties are electrophoretic homogeneity at pH 4.0 and its transformation to an electrophoretically heterogeneous form (2). In continuation of this study, the question was asked whether the ordinary human serum albumin (Fraction V), known to bc electrophoretically heterogeneous at pH 4...
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Human serum albumin (HSA), under conditions of low pH, is known to exist in two isomeric forms, the F form at around pH 4.0 and the E form below 3.0. We studied its conformation in the acid-denatured E form using far-UV and near-UV CD, binding of a hydrophobic probe, 1-anilinonaphthalene-8-sulfonic acid (ANS), thermal transition by far-UV and near-UV CD, tryptophan fluorescence, quenching of tr...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1958
ISSN: 0306-3283
DOI: 10.1042/bj0690627